Combined transcriptome and metabolite profiling reveals that IiPLR1 plays an important role in lariciresinol accumulation in Isatis indigotica.
Identifieur interne : 000433 ( PubMed/Checkpoint ); précédent : 000432; suivant : 000434Combined transcriptome and metabolite profiling reveals that IiPLR1 plays an important role in lariciresinol accumulation in Isatis indigotica.
Auteurs : Ying Xiao [République populaire de Chine] ; Qian Ji [République populaire de Chine] ; Shouhong Gao [République populaire de Chine] ; Hexin Tan [République populaire de Chine] ; Ruibing Chen [République populaire de Chine] ; Qing Li [République populaire de Chine] ; Junfeng Chen [République populaire de Chine] ; Yingbo Yang [République populaire de Chine] ; Lei Zhang [République populaire de Chine] ; Zhengtao Wang [République populaire de Chine] ; Wansheng Chen [République populaire de Chine] ; Zhibi Hu [République populaire de Chine]Source :
- Journal of experimental botany [ 1460-2431 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Isatis, Protéines végétales.
- métabolisme : Furanes, Isatis, Lignanes, Protéines végétales.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Phylogénie, Transcriptome.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Furans, Lignans, Plant Proteins.
- genetics : Isatis.
- metabolism : Isatis.
- Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Transcriptome.
Abstract
A lignan, lariciresinol, is an important efficacious compound for the antiviral effect of Isatis indigotica, a widely used herb for the treatment of colds, fever, and influenza. Although some rate-limiting steps of the lariciresinol biosynthetic pathway are well known, the specific roles of gene family members in I. indigotica in regulating lariciresinol production are poorly understood. In the present study, a correlation analysis between the RNA sequencing (RNA-Seq) expression profile and lignan content by using I. indigotica hairy roots treated with methyl jamonate (0.5 μM) at different time points as a source implicated that I. indigotica pinoresinol/lariciresinol reductase 1 (IiPLR1), but not IiPLR2 or IiPLR3, contributed greatly to lariciresinol accumulation. Gene silencing by RNA interference (RNAi) demonstrated that IiPLR1 indeed influenced lariciresinol biosynthesis, whereas suppression of IiPLR2 or IiPLR3 did not change lariciresinol abundance significantly. IiPLR1 was thus further characterized; IiPLR1 was constitutively expressed in roots, stems, leaves, and flowers of I. indigotica, with the highest expression in roots, and it responds to different stress treatments to various degrees. Recombinant IiPLR1 reduces both (±)-pinoresinol and (±)-lariciresinol efficiently, with comparative K cat/K m values. Furthermore, overexpression of IiPLR1 significantly enhanced lariciresinol accumulation in I. indigotica hairy roots, and the best line (ovx-2) produced 353.9 μg g(-1) lariciresinol, which was ~6.3-fold more than the wild type. This study sheds light on how to increase desired metabolites effectively by more accurate or appropriate genetic engineering strategies, and also provides an effective approach for the large-scale commercial production of pharmaceutically valuable lariciresinol by using hairy root culture systems as bioreactors.
DOI: 10.1093/jxb/erv333
PubMed: 26163698
Affiliations:
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of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003</wicri:regionArea><wicri:noRegion>Shanghai 200003</wicri:noRegion></affiliation></author><author><name sortKey="Ji, Qian" sort="Ji, Qian" uniqKey="Ji Q" first="Qian" last="Ji">Qian Ji</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.</nlm:affiliation><country 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Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433</wicri:regionArea><wicri:noRegion>Shanghai 200433</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Ruibing" sort="Chen, Ruibing" uniqKey="Chen R" first="Ruibing" last="Chen">Ruibing Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433</wicri:regionArea><wicri:noRegion>Shanghai 200433</wicri:noRegion></affiliation></author><author><name sortKey="Li, Qing" sort="Li, Qing" uniqKey="Li Q" first="Qing" last="Li">Qing Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003</wicri:regionArea><wicri:noRegion>Shanghai 200003</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Junfeng" sort="Chen, Junfeng" uniqKey="Chen J" first="Junfeng" last="Chen">Junfeng Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003</wicri:regionArea><wicri:noRegion>Shanghai 200003</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Yingbo" sort="Yang, Yingbo" uniqKey="Yang Y" first="Yingbo" last="Yang">Yingbo Yang</name><affiliation wicri:level="1"><nlm:affiliation>The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203</wicri:regionArea><wicri:noRegion>Shanghai 201203</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Lei" sort="Zhang, Lei" uniqKey="Zhang L" first="Lei" last="Zhang">Lei Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China zhanglei@smmu.edu.cn ztwang@shutcm.edu.cn chenwansheng@smmu.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433</wicri:regionArea><wicri:noRegion>Shanghai 200433</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Zhengtao" sort="Wang, Zhengtao" uniqKey="Wang Z" first="Zhengtao" last="Wang">Zhengtao Wang</name><affiliation wicri:level="1"><nlm:affiliation>The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China zhanglei@smmu.edu.cn ztwang@shutcm.edu.cn chenwansheng@smmu.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203</wicri:regionArea><wicri:noRegion>Shanghai 201203</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Wansheng" sort="Chen, Wansheng" uniqKey="Chen W" first="Wansheng" last="Chen">Wansheng Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China zhanglei@smmu.edu.cn ztwang@shutcm.edu.cn chenwansheng@smmu.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003</wicri:regionArea><wicri:noRegion>Shanghai 200003</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Zhibi" sort="Hu, Zhibi" uniqKey="Hu Z" first="Zhibi" last="Hu">Zhibi Hu</name><affiliation wicri:level="1"><nlm:affiliation>The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203</wicri:regionArea><wicri:noRegion>Shanghai 201203</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Furans (metabolism)</term><term>Isatis (genetics)</term><term>Isatis (metabolism)</term><term>Lignans (metabolism)</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Sequence Analysis, DNA</term><term>Transcriptome</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Furanes (métabolisme)</term><term>Isatis (génétique)</term><term>Isatis (métabolisme)</term><term>Lignanes (métabolisme)</term><term>Phylogénie</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Transcriptome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Furans</term><term>Lignans</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Isatis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Isatis</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Isatis</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Furanes</term><term>Isatis</term><term>Lignanes</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Transcriptome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Phylogénie</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A lignan, lariciresinol, is an important efficacious compound for the antiviral effect of Isatis indigotica, a widely used herb for the treatment of colds, fever, and influenza. Although some rate-limiting steps of the lariciresinol biosynthetic pathway are well known, the specific roles of gene family members in I. indigotica in regulating lariciresinol production are poorly understood. In the present study, a correlation analysis between the RNA sequencing (RNA-Seq) expression profile and lignan content by using I. indigotica hairy roots treated with methyl jamonate (0.5 μM) at different time points as a source implicated that I. indigotica pinoresinol/lariciresinol reductase 1 (IiPLR1), but not IiPLR2 or IiPLR3, contributed greatly to lariciresinol accumulation. Gene silencing by RNA interference (RNAi) demonstrated that IiPLR1 indeed influenced lariciresinol biosynthesis, whereas suppression of IiPLR2 or IiPLR3 did not change lariciresinol abundance significantly. IiPLR1 was thus further characterized; IiPLR1 was constitutively expressed in roots, stems, leaves, and flowers of I. indigotica, with the highest expression in roots, and it responds to different stress treatments to various degrees. Recombinant IiPLR1 reduces both (±)-pinoresinol and (±)-lariciresinol efficiently, with comparative K cat/K m values. Furthermore, overexpression of IiPLR1 significantly enhanced lariciresinol accumulation in I. indigotica hairy roots, and the best line (ovx-2) produced 353.9 μg g(-1) lariciresinol, which was ~6.3-fold more than the wild type. This study sheds light on how to increase desired metabolites effectively by more accurate or appropriate genetic engineering strategies, and also provides an effective approach for the large-scale commercial production of pharmaceutically valuable lariciresinol by using hairy root culture systems as bioreactors. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26163698</PMID><DateCompleted><Year>2016</Year><Month>07</Month><Day>19</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>66</Volume><Issue>20</Issue><PubDate><Year>2015</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J. Exp. Bot.</ISOAbbreviation></Journal><ArticleTitle>Combined transcriptome and metabolite profiling reveals that IiPLR1 plays an important role in lariciresinol accumulation in Isatis indigotica.</ArticleTitle><Pagination><MedlinePgn>6259-71</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/erv333</ELocationID><Abstract><AbstractText>A lignan, lariciresinol, is an important efficacious compound for the antiviral effect of Isatis indigotica, a widely used herb for the treatment of colds, fever, and influenza. Although some rate-limiting steps of the lariciresinol biosynthetic pathway are well known, the specific roles of gene family members in I. indigotica in regulating lariciresinol production are poorly understood. In the present study, a correlation analysis between the RNA sequencing (RNA-Seq) expression profile and lignan content by using I. indigotica hairy roots treated with methyl jamonate (0.5 μM) at different time points as a source implicated that I. indigotica pinoresinol/lariciresinol reductase 1 (IiPLR1), but not IiPLR2 or IiPLR3, contributed greatly to lariciresinol accumulation. Gene silencing by RNA interference (RNAi) demonstrated that IiPLR1 indeed influenced lariciresinol biosynthesis, whereas suppression of IiPLR2 or IiPLR3 did not change lariciresinol abundance significantly. IiPLR1 was thus further characterized; IiPLR1 was constitutively expressed in roots, stems, leaves, and flowers of I. indigotica, with the highest expression in roots, and it responds to different stress treatments to various degrees. Recombinant IiPLR1 reduces both (±)-pinoresinol and (±)-lariciresinol efficiently, with comparative K cat/K m values. Furthermore, overexpression of IiPLR1 significantly enhanced lariciresinol accumulation in I. indigotica hairy roots, and the best line (ovx-2) produced 353.9 μg g(-1) lariciresinol, which was ~6.3-fold more than the wild type. This study sheds light on how to increase desired metabolites effectively by more accurate or appropriate genetic engineering strategies, and also provides an effective approach for the large-scale commercial production of pharmaceutically valuable lariciresinol by using hairy root culture systems as bioreactors. </AbstractText><CopyrightInformation>© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ji</LastName><ForeName>Qian</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Shouhong</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Hexin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Ruibing</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Qing</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Junfeng</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yingbo</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Lei</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China zhanglei@smmu.edu.cn ztwang@shutcm.edu.cn chenwansheng@smmu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Zhengtao</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China zhanglei@smmu.edu.cn ztwang@shutcm.edu.cn chenwansheng@smmu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Wansheng</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China zhanglei@smmu.edu.cn ztwang@shutcm.edu.cn chenwansheng@smmu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Zhibi</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>07</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005663">Furans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017705">Lignans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>73XCE5OZB0</RegistryNumber><NameOfSubstance UI="C060282">lariciresinol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005663" MajorTopicYN="N">Furans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031219" MajorTopicYN="N">Isatis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017705" MajorTopicYN="N">Lignans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="Y">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Correlation analysis</Keyword><Keyword MajorTopicYN="N">Isatis indigotica</Keyword><Keyword MajorTopicYN="N">RNA-Seq expression profile.</Keyword><Keyword MajorTopicYN="N">lariciresinol</Keyword><Keyword MajorTopicYN="N">lignan</Keyword><Keyword MajorTopicYN="N">pinoresinol/lariciresinol reductase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>7</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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